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Expression of genes for metabolic enzymes of cinnamic acid and its derivatives in potato leaves under conditions of late blight infection

Abstract

An analysis of changes in the expression of genes encoding enzymes for the metabolism of cinnamic acid and its derivatives was carried out in the leaf cells of potato varieties of Belarusian selection with different levels of resistance to late blight under conditions of 3-day infection with the pathogen. Differences in the expression profiles of the studied genes were revealed depending on the resistance of the studied varieties to late blight and the duration of infection.

About the Authors

A. A. Smirnov
ГНУ «Институт биофизики и клеточной инженерии НАН Беларуси»
Russian Federation


E. M. Kabachevskaya
ГНУ «Институт биофизики и клеточной инженерии НАН Беларуси»
Belarus


I. I. Busko
РУП «Научно-практический центр Национальной академии наук Беларуси по картофелеводству и плодоовощеводству»
Belarus


I. D. Volotovskiy
ГНУ «Институт биофизики и клеточной инженерии НАН Беларуси»
Belarus


References

1. Aharoni, A. Metabolic engineering of the plant primary-secondary metabolism interface / A. Aharoni, G. Galili // Curr. Opin. Biotechnol. – 2011. – № 22. – P. 239–244.

2. Zhang, X. Multifaceted regulations of gateway enzyme phenylalanine ammonia-lyase in the biosynthesis of phenylpropanoids / X. Zhang, C. J. Liu // Mol. Plant. – 2015. – № 8. – P. 17–27.

3. Role of phenolic compounds in plant-defensive mechanisms. In: Plant Phenolics in Sustainable Agriculture / S. Kumar [et al.]. – Singapore: Springer, 2020. – P. 517–532.

4. Jones, D. H. Phenylalanine ammonia-lyase. Regulation of its induction, and its role in plant development / D. H. Jones // Phytochemistry. – 1984. – № 23. – P. 1349–1359.

5. Abnormal Plant Development and Down-Regulation of Phenylpropanoid Biosynthesis in Transgenic Tobacco Containing a Heterologous Phenylalanine Ammonia-Lyase Gene / Y. Elkind [et al.] // Proc. Nadl. Acad. Sci. USA. – 1990. – № 87 (22). – P. 9057–9061.

6. Increased disease susceptibility of transgenic tobacco plants with suppressed levels of preformed phenylpropanoid products / E. A. Maher [et al.] // Proc. Natl. Acad. Sci. USA. – 1994. – №91. – P. 7802–7806.

7. Wei, K. Global identification, structural analysis and expression characterization of cytochrome P450 monooxygenase superfamily in rice / K. Wei, H. Chen // BMC Genomics. – 2018. – № 19 (1). – P. 35.

8. Genome-wide characterization of the lignification toolbox in Arabidopsis / J. Raes [et al.] // Plant Physiol. – 2003. – № 133. – P. 1051–1071.

9. Facchini, P. J. Hydroxycinnamic acid amide metabolism: Physiology and biochemistry / P. J. Facchini, J. Hagel, K. G. Zulak // Can. J. Bot. – 2002. – № 80. – P. 577–589.

10. Transgenic tomato plants overexpressing tyramine N-Hydroxycinnamoyltransferase exhibit elevated hydroxycinnamic acid amide levels and enhanced resistance to Pseudomonas syringae / L. Campos [et al.] // MPMI. – 2014. – V. 27. – P. 1159–1169.

11. Hohlfeld, H. Purification of hydroxycinnamoyl-CoA: tyramine hydroxycinnamoyltransferase from cell-suspension cultures of Solanum tuberosum L. / H. Hohlfeld, D. Scheel, D. Strack // Planta (Heidelb.). – 1996. – № 199. – P. 166–168.

12. A novel polyamine acyltransferase responsible for the accumulation of spermidine conjugates in Arabidopsis seed / J. Luo [et al.] // Plant Cell. – 2009. – № 21(1). – P. 318–333.

13. Базальный уровень экспрессии генов ферментов метаболизма коричной кислоты в листьях растений картофеля in vitro сортов белорусской селекции / Е. М. Кабачевская [и др.] // Картофелеводство: сб. науч. тр. / Науч.-практ. центр Нац. акад. Наук Беларуси по картофелеводству и плодоовощеводству; редкол. : В. Л. Маханько (гл. ред.) [и др.]. – Минск, 2020. – Т. 28. – С. 51–55.

14. Michael, W. New mathematical model for relative quantification in real-time RT–PCR / W. Michael, A. Pfaffl // Nucleic Acids Research. – 2001. – V. 29, № 9. – P. 45.

15. Pfaffl, M.W. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR / M. W. Pfaffl, G. W. Horgan, L. Dempfle // Nucleic Acids Res. – 2002. – V. 30, № 9. – P. 36.

16. Multiple R genes and phenolic compounds synthesis involved in the durable resistance to Phytophthora infestans in potato cv. Cooperation 88 / Dahai Hao [et al.] // Agri. Gene. – 2018. – V. 8. – P. 28–36.

17. Cooperation-88: A High Yielding, Multi-Purpose, Late Blight Resistant Cultivar Growing in Southwest China / C. Li [et al.] // Am. J. Pot. Res. – 2011. – Vol. 88. – P. 190–194.


Review

For citations:


Smirnov A.A., Kabachevskaya E.M., Busko I.I., Volotovskiy I.D. Expression of genes for metabolic enzymes of cinnamic acid and its derivatives in potato leaves under conditions of late blight infection. Potato and Vegetable Growing. 2023;1:228-237. (In Russ.)

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